Fig. 5.

Assessment of the post-thawing recovery of L929 cells. MTS assay (A) and DNA Assay (B) after 24 h, 48 h, and 72 h of culture. Data represent means ± SD (n = 3). Statistically significant differences were determined by Tukey's multiple comparisons test and are represented in asterisks, *p < 0.05 **p < 0.02; ***p < 0.001 and ****p = 0.0001, two-way ANOVA. The absence of asterisks means that there are no significant differences between NADES and between NADES and Me₂SO at the corresponding time points. Post-thawing DAPI/Phalloidin assay (cells nuclei were stained blue by DAPI and F-actin filaments in red by phalloidin) (C1), Live/Dead fluorescence assay (living cells were stained green by calcein AM and dead cells red by PI) (C2) and Optical microscopy (C3) of L929 cells at 24 h and 72 h.