Figure 3.

Cryo-EM structure of WT KatG^INH

(A) Cryo-EM model of KatG^INH in two orientations. Protomer A is shown in yellow with the N-terminal domain (NTD) a darker shade than the C-terminal domain (CTD). Protomer B is shown in cyan and heme in red. The N-terminal hook is highlighted in the dashed red circle.

(B) Linear schematic of WT KatG with the NTD and CTD colored according to protomer A in (A). Conserved distal and proximal site residues are indicated, and the covalent catalytic triad labeled.

(C) The heme environment with distal and proximal heme site residues shown as purple sticks and heme in red.

(D) The catalytic triad formed by the MYW (Met255-Tyr229-Trp107) residues. Cryo-EM maps are shown as gray mesh.