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. 2021 Aug 5;29(8):899–912.e4. doi: 10.1016/j.str.2020.12.008

Figure 7.

Figure 7

Addition of hemin chloride to T275P, WT, and W107R KatG protein

(A) Changes in the UV-visible spectrum of the T275P variant (3.3 μM) upon the addition of hemin chloride. The arrow indicates the absorbance increase upon addition of hemin chloride. Inset displays the absorbance at 396 nm plotted against heme concentration with a hyperbolic fit.

(B) Corrected UV-visible absorbance spectrum of T275P KatG variant protein (see the STAR methods) following the addition of hemin chloride. Inset displays the corrected absorbance at 396 nm plotted against heme concentration with lines to indicate stoichiometry. The downward slope at higher free heme concentrations is due to the non-linearity of the absorbance of heme as a function of concentration (see the STAR methods and Figure S6).

(C) WT KatG protein identical to (A).

(D) Corrected UV-visible absorbance spectrum of WT KatG identical to (B).

(E) W107R KatG variant protein identical to (A and C).

(F) Corrected UV-visible absorbance spectrum of W107R KatG variant protein identical to (B and D). All at 20°C in 20 mM NaPi, pH 7, 100 mM NaCl.