REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
B21(DE3) E. coli	Lab stock	N/A
Chemicals, Peptides, and Recombinant Proteins
KatG recombinant protein	Lab	N/A
Hemin chloride	Sigma-Aldrich	H9039
5-Aminolevulinic acid hydrochloride	Sigma-Aldrich	A3785
Hydrogen Peroxide Solution	Sigma-Aldrich	H1009
Deposited Data
WT KatG structure	This paper	PDB: 6ZJI
WT KatG Cryo-EM map	This paper	EMDB: 11234
WT KatG +INH structure	This paper	PDB: 7AG8
WT KatG +INH Cryo-EM map	This paper	EMDB: 11776
T275P structure	This paper	PDB: 7A8Z
T275P Cryo-EM map	This paper	EMDB: 11680
T275P +Heme structure	This paper	PDB: 7AA3
T275P +Heme Cryo-EM map	This paper	EMDB: 11689
W107R structure	This paper	PDB: 7A2I
W107R Cryo-EM map	This paper	EMDB: 11625
W107R + Heme (1 heme) structure	This paper	PDB: 7A7C
W107R + Heme (1 heme) Cryo-EM map	This paper	EMDB: 11677
W107R + Heme (2 heme) structure	This paper	PDB: 7A7A
W107R + Heme (2 heme) Cryo-EM map	This paper	EMDB: 11676
The crystal structure of KatG from Mycobacterium tuberculosis.	Zhao et al., 2006	PDB: 2CCA
The crystal structure of Isoniazid bound KatG from Synechococcus elongatus.	Kamachi et al., 2015a, 2015b	PDB: 3WXO
Oligonucleotides
T275P KatG Forward primer 5’-ACCTTTGGTAAACCACATGGTGCAGGT-3’	This paper	N/A
T275P KatG Reverse primer 5’-ACCTGCACCATGTGGTTTACCAAAGGT-3’	This paper	N/A
W107R KatG Forward primer 5’-TGCTGCATGCCGTGCCATACGAATAAACAGCGG-3’	This paper	N/A
W107R KatG Reverse primer 5’-CCGCTGTTTATTCGTATGGCACGGCATGCAGCA-3’	This paper	N/A
Recombinant DNA
W107R – pET28a plasmid	This paper	N/A
T275P – pET28a plasmid	This paper	N/A
WT – pET28a plasmid	This paper	N/A
Software and Algorithms
Warp	Tegunov and Cramer, 2019	http://www.warpem.com/warp/
CryoSPARC	Punjani et al., 2017a, Punjani et al., 2017b	https://cryosparc.com
Coot	Emsley et al., 2010	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PHENIX Real-space refinement	Afonine et al., 2018b	https://www.phenix-online.org/documentation/reference/real_space_refine.html
Fragment Hotspot Maps	Radoux et al., 2016	http://fragment-hotspot-maps.ccdc.cam.ac.uk/
Namdinator	Kidmose et al., 2019	https://namdinator.au.dk
UCSF chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/download.html