Fig. 1. Immunization experiments with the stabilized BG505 SOSIP trimers.

A Immunogen (left) and immunization schedule (right). B ELISA binding titers (midpoint) and C neutralizing antibody titers (ID₅₀) for plasma samples collected at weeks 10, 26, and 38 (time points indicated above each graph; open circles—Grp 1; closed circles—Grp 2). Horizontal lines represent the geometric mean values at each time point (n = 6 animals in each group). ELISA and pseudovirus neutralization experiments were performed in triplicates (n = 3) for each serum sample at every time point and the mean value is plotted. D Composite figures from nsEMPEM analysis of polyclonal responses at week 26. Animal IDs and neutralizing antibody titers (at week 26) for corresponding animals are shown above and below each composite figure, respectively. A color-coding scheme for antibodies targeting different epitope clusters is shown at the bottom. BG505 SOSIP antigen is represented in gray.