Table 3.

Metrics of genetic diversity across 8 populations of Diadumene lineata for the (A) genet-level (no clones) and (B) ramet-level (clones included) data sets

(A)	Region	Site	MAWT	Genets	A  E	P  A	HE	H  O	${\overline{r}}_D$	F  IS
	FL	CML	22.4	3	2.53 ± 0.20	0.08 ± 0.05	0.673 ± 0.066	0.533 ± 0.102	0.042	0.247*
	FL	WAK	22.4	2	2.80 ± 0.25	0.35 ± 0.15	0.8 ± 0.042	0.700 ± 0.133	—	0.176
	GA	STS	20.9	5	2.60 ± 0.15	0.25 ± 0.1	0.691 ± 0.049	0.680 ± 0.104	0.236***	0.018
	GA	JEK	20.9	7	2.44 ± 0.22	0.36 ± 0.19	0.613 ± 0.074	0.557 ± 0.105	0.239***	0.098
	VA	RBB	16.1	6	2.46 ± 0.15	0.26 ± 0.11	0.652 ± 0.059	0.767 ± 0.09	0.207***	−0.198*
	VA	QBY	16.1	7	2.46 ± 0.19	0.29 ± 0.12	0.643 ± 0.063	0.586 ± 0.116	0.216**	0.096
	MA	NAH	12.8	5	2.74 ± 0.22	0.31 ± 0.08	0.713 ± 0.071	0.600 ± 0.084	0.093	0.175*
	MA	PMH	12.8	4	2.50 ± 0.25	0.44 ± 0.11	0.643 ± 0.087	0.725 ± 0.126	0.485***	−0.152
				Mean ± SE	2.57 ± 0.16	0.29 ± 0.07	0.679 ± 0.023	0.643 ± 0.038	0.064***	0.06 ± 0.06
(B)	Region	Site	MAWT	N	A  E	P  A	H  E	H  O	${\overline{r}}_D$	F  IS
	FL	CML	22.4	25	3.00 ± 0.3	0 ± 0	0.507 ± 0.049	0.520 ± 0.12	0.807***	−0.026
	FL	WAK	22.4	26	2.67 ± 0.21	0.21 ± 0.13	0.442 ± 0.061	0.792 ± 0.132	1.000***	−0.823***
	GA	STS	20.9	27	3.61 ± 0.31	0.11 ± 0.1	0.455 ± 0.058	0.696 ± 0.134	0.763***	−0.546***
	GA	JEK	20.9	23	4.35 ± 0.67	0.61 ± 0.39	0.515 ± 0.073	0.535 ± 0.119	0.610***	−0.04
	VA	RBB	16.1	30	3.43 ± 0.27	0.17 ± 0.12	0.559 ± 0.061	0.800 ± 0.101	0.583***	−0.441***
	VA	QBY	16.1	28	3.98 ± 0.57	0.32 ± 0.23	0.570 ± 0.062	0.564 ± 0.116	0.494***	0.01
	MA	NAH	12.8	27	4.45 ± 0.53	0.48 ± 0.16	0.604 ± 0.066	0.563 ± 0.091	0.652***	0.07
	MA	PMH	12.8	21	3.5 ± 0.52	0.50 ± 0.22	0.587 ± 0.08	0.710 ± 0.126	0.702***	−0.215***
				Mean ± SE	3.62 ± 0.34	0.30 ± 0.11	0.530 ± 0.023	0.648 ± 0.042	0.248***	−0.25 ± 0.12

MAWT, mean annual water temperature in Celsius; genet, number of genotypes based on P_sex; N, number of anemones genotyped; A_E, allelic richness based on rarefaction using the smallest samples size (ramet level: 21 anemones, 42 alleles; genet level: 2 anemones, 4 alleles); P_A, private allelic richness based on rarefaction using the smallest samples size (ramet level: 21 anemones, 42 alleles; genet level: 2 anemones, 4 alleles); H_E, unbiased expected heterozygosity; H_O, observed heterozygosity; ${\overline{r}}_D$, linkage disequilibrium, F_IS, inbreeding coefficient and test for deviation from Hardy–Weinberg expectations performed using 1000 permutations among individuals within samples using FSTAT (Goudet 1995). SE, standard error.

Significant P-values are shown in bold with an * for P < 0.05, ** for P < 0.01, *** for P < 0.001. For F_IS, the Bonferroni adjusted α = 0.00063, ^#P < 0.00063.