. 2020 Dec 12;99(1):skaa394. doi: 10.1093/jas/skaa394

Table 4.

Methane (CH₄) and hydrogen (H₂) emissions of steers fed a corn-based finishing diet without (control) or supplemented with low, medium, and high doses 3-NOP that met the criteria for measuring gaseous emissions

	Treatment¹					P-value²
Item	Control	Low	Medium	High	SEM	3-NOP	Period	Int
CH43
g/d	126.4	54.8	28.6	41.2	14.94	<0.001	0.62	0.020
Period 1	139.6^a	48.4^b	22.7^b	28.9^b
Period 2	124.1^a	50.4^b	32.9^b	56.4^b
Period 3	115.4^a	65.6^b	30.2^c	38.3^c
g/kg total DMI	11.32	5.41	2.72	4.16	1.77	<0.001	0.60	0.048
Period 1	12.13^a	4.95^b	2.32^b	2.65^b
Period 2	11.35^a	4.64^b	3.06^b	5.88^b
Period 3	10.47^a	6.65^b	2.79^c	3.94^c
% GE intake	3.42	1.63	0.82	1.26	0.53	<0.001	0.60	0.048
Period 1	3.66^a	1.49^b	0.70^b	0.80^b
Period 2	3.43^a	1.40^b	0.92^bc	1.78^b
Period 3	3.16^a	2.01^b	0.84^c	1.19^c
H₂
g/d	0.53	2.42	2.62	2.61	0.22	<0.001	<0.001	0.004
Period 1	0.60^c	2.81^b	3.05^ab	3.26^a
Period 2	0.56^b	2.66^a	2.62^a	2.48^a
Period 3	0.41^c	1.80^b	2.18^a	2.08^ab
g/kg total DMI	0.049	0.231	0.229	0.243	0.01	<0.001	<0.001	<0.001
Period 1	0.054^b	0.274^a	0.272^a	0.308^a
Period 2	0.053^b	0.242^a	0.231^a	0.226^a
Period 3	0.039^b	0.176^a	0.185^a	0.194^a

¹The measured 3-NOP concentration was 0, 85.6, 107.6, and 124.5 mg/kg dietary DM consumed for the control, low, medium, and high treatment, respectively.

²Int, interaction between 3-NOP and period.

³Only animals with ≥10 “good” weekly visits in at least five of the six 4-h time blocks were selected for final analysis. Useful/good visits were selected based on the distance of the animal’s head from the proximity sensor and the duration that the animal’s head in the “head chamber.”

^a–cValues within a row with different letters differ at P ≤ 0.05.