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. 2021 Jul 28;12:716661. doi: 10.3389/fimmu.2021.716661

Figure 5.

Figure 5

(A–D) Scatter plots show correlation between (left) glycolytic enzyme gene expression and basal glycolysis (ECAR), or (right) correlation between electron transport chain gene expression and basal respiration (OCR) in (A) M-THY, (B) M-ATO, and (C) H-THY, and (D) H-ATO. The x axis represents the Pearson correlation coefficient R values calculated for individual genes within the specified metabolic pathway for all cell populations for which respective extracellular flux data was collected. The y axis represents the statistical significance of each R value using -log10 of Benjamini-Hochberg adjusted p-values. Solid black line indicates an adjusted p-value of 0.05; all R values above the line are statistically significant. Genes with R values >0.5 (pink) are positively correlated with metabolic flux data, while genes with R values <-0.5 (blue) are negatively correlated with metabolic flux data. Nonsignificant or weak correlation R values >-.05 or <0.5 are indicated in grey. Certain genes of interest are indicated. (M-THY: n=6 flux analysis values for 5 populations, n=2 RNA-seq replicates; H-THY: n=3 flux analysis values for 5 populations, n=3 RNA-seq replicates; M-ATO: n=6 flux analysis values for 3 populations, n=2 RNA-seq replicates; H-ATO: n=6 flux analysis values for 3 populations, n=2 RNA-seq replicates).