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. 2021 Jul 28;12:716661. doi: 10.3389/fimmu.2021.716661

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Copyright © 2021 Sun, Sharpley, Kaczor-Urbanowicz, Chang, Montel-Hagen, Lopez, Zampieri, Zhu, de Barros, Parekh, Casero, Banerjee and Crooks

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Representative flow cytometry analysis of thymocyte populations from Week 3 and 5 M-ATOs derived from WT and Rag1^-/- mouse bone marrow. (B) Extracellular flux analyses of thymocyte populations from Week 3 M-ATOs derived from WT or Week 5 M-ATOs derived from Rag1^-/- mouse bone marrow HSPCs. (C) Comparison of basal ECAR, basal OCR, maximal OCR, between thymocyte populations from M-ATOs derived from WT or Rag1^-/- bone marrow HSPCs. (WT: n=6, Rag1^-/-: n=3 independent experiments). (error bars: sd, significance: multiple t-test p values, comparisons between WT and Rag1^-/- ATO populations denoted by *; comparisons between Rag1^-/- ATO populations denoted by . * or ^#p < 0.05; ** or ^##p < 0.01; *** or ^###p < 0.001).