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. 2021 Jul 28;12:711701. doi: 10.3389/fphar.2021.711701

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Copyright © 2021 Xue, Zhang, Liu, Liu, Li, Han, Sun and Chu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of 8-Gin on the expression of Caspase-9, Bax, Bcl-2 protein. (A) Representative images of Caspase-9, Bax, Bcl-2 protein expression that were exhibited from cardiac tissue in the CON, ISO, 8-Gin_L + ISO, 8-Gin_H + ISO, and CAP + ISO groups. (B–D) Pooled analysis of Caspase-9, Bax, Bcl-2 protein expression by western blot analysis on which intensity was normalized to GAPDH. (E) The ratio of Bax and Bcl-2 protein expression by western blot analysis. Data are shown as the mean ± SEM, n = 3. ^* p < 0.05, ^** p < 0.01 vs. the CON group; ^# p < 0.05, ^## p < 0.01 vs. the ISO group.