Table 2.
Gene ID, sequence, and accession number for all genes assessed in the testes of 12-wk-old Holstein Friesian bull calves offered a high (HI) or moderate (MOD) plane of nutrition from 2 to 12 wk of life
| Gene1 | Primer sequence (5′–3′) | Accession number |
|---|---|---|
| FSHR | FWD: CGCTGGAAAGATGGCATACC RVS: GCTCACCTTCATGTAGCTGC |
NM_174061.1 |
| LHR | FWD: GGGAAATCAGCGTTGTCCCATTGA RVS: GCATCCACAAGCTTCCAGATGTTACGA |
NM_174381.1 |
| AR | FWD: AGCCTCAATGAACTGGGTGA RVS: AACACCATAAGCCCCATCCA |
NM_001244127.1 |
| IGF1R | FWD: TTTCTCAATGAGGCCTCGGT RVS: CTGCAATCTCACCAGCCATC |
NM_001244612.1 |
| PCNA | FWD: GGCGTTCATAGTCGTGTTCC RVS: CTTCAAGATGGAGCCCTGGA |
NM_001034494.1 |
| StAR | FWD: GCCAGGAAAGATGCTTCTCG RVS: GACGCCGAACCTGGTTAATC |
NM_174189.3 |
| INSL3 | FWD: GTGAACTCCTGATGCCACAC RVS: TTTGGGGTCTGGTGGTATCC |
NM_174365.2 |
| CLDN11 | FWD: GCCTCTCTGTTCCCTCTCTC RVS: CCCACCCCACATTCCCTATT |
NM_001035055.2 |
| THY1 | FWD: CCTCACCTCTGCCAATACCA RVS: ACGGAAGCAGCTCTGGAATA |
NM_001034765.1 |
| UCHL1 | FWD: GCTTCTCTGGGTTGTGTTCG RVS: GGTCAGCACTTTGTTCAGCA |
NM_001046172.2 |
| IGF-1 | FWD: ATGCCCAAGGCTCAGAAG RVS: GGTGGCATGTCATTCTTCACT |
NM_001077828 |
| GHR | FWD: ATGGCGGTATTGTGGATCAT RVS: AGGATGTCGGCATGAATCTC |
NM_176608.1 |
| FOS | FWD: TTTGACTGCTCGCGATCATG RVS: CAGATCGGTGCAGTAGTCCT |
NM_182786 |
| SIRT1 | FWD: CCAACGGTTTCCATTCGTGT RVS: TCGAGGATCTGTGCCAATCA |
NM_001192980.1 |
| PIK3 | FWD: AAATGCGGCGACCAGATTTC RVS: AAGCCTGAGATTTCCCAGCT |
NM_174574.1 |
| EIF4E | FWD: AATGCCTGGCTGTGACTACT RVS: ATTAGCCATCGTCCTCCTCG |
NM_174310.3 |
| MTOR | FWD: CACAAGTCGAGCTGCTCATC RVS: CTGTGCCTCCAGTTACCAGA |
XM_015466779.1 |
| EIFEBP1 | FWD: CCCCTTCTTCTTCGTGGACA RVS: CTCTTCTGTGTCCACTTGCC |
NM_001077893.2 |
| PRKAA1 | FWD: TGTCACAGGCATATGGTGGT RVS: GTGCAGCATAGTTGGGTGAG |
NM_001109802.2 |
| INSR | FWD: TGAAGCCAAGGCAGATGATATT RVS: GCCACATCAAGTGAACAACGTT |
XM_590552.4 |
| AKT | FWD: ACATCCGAGACCAGTCCAAG RVS: TGTCGAGTGTGTCTGCGTAT |
NM_001191309 |
| EBP | FWD: TGGTACCCGGACTCCTCATA RVS: AGCCGGTGTTTAAGCCTAGT |
NM_001034500.1 |
| GAPDH | FWD: AGATGGTGAAGGTCGGAGTG RVS: TGACTGTGCCGTTGAACTTG |
NM_001034034.2 |
| UBQ | FWD: CACGTTTGGGAGTTGTGCTT RVS: TCTCTTCCACCATTCCCACC |
NM_001206307 |
| YWHAZ | FWD: GATCCCCAACGCTTCACAAG RVS: TTGTCATCACCAGCTGCAAC |
NM_174814.2 |
| RPS9 | FWD: GCTGACGCTGGATGAGAAAG RVS: ATCCAGCTTCATCTTGCCCT |
NM_001101152.2 |
1AKT, serine/threonine kinase 1; AMH, anti-Müllerian hormone; AR, androgen receptor; CLDN11, claudin 11; EBP, cholestenol delta-isomerase; EIF4E, eukaryotic translation initiation factor 4E; EIFEBP1, eukaryotic translation initiation factor 4E-binding protein 1; FOS, Fos proto-oncogene, AP-1 transcription factor subunit; FSHR, follicle-stimulating hormone receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GATA4, GATA binding protein 4; GHR, growth hormone receptor; IGF-1, insulin-like growth factor 1; IGF1-R, insulin-like growth factor 1 receptor; INSL3, insulin-like 3; INSR, insulin receptor; LHR, luteinising hormone receptor; Mtor, mechanistic target of rapamycin kinase; MVK, mevalonate kinase; PCNA, proliferation cell nuclear antigen; PIK3, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma; PRKAA1, protein kinase AMP-activated catalytic subunit alpha 1; RPS9, ribosomal protein S9; StAR, steroidogenic acute regulatory protein; THY1, Thy-1 cell surface antigen; UBQ, ubiquitin; UCHL1, ubiquitin carboxyl-terminal esterase L1; YWHAZ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta.