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. 2021 Jul 28;12:732540. doi: 10.3389/fphar.2021.732540

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Copyright © 2021 Liu, Ding, Hou, Yu, Nie and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of miR-130a-3p on the differentiation in TGF-β1-induced MLg. (A) The relative cell viability was measured in CCK8 assay, and the data for NC group was set to 100%. (B) The effect of miR-130a-3p on fibroblast-myofibroblast transition was measured with immunofluorescence. Scale bar = 10 μm. (C) Western blot bands of α-SMA and FN protein expression in MLg after transfection of miR-130a-3p. (D,E) Statistical analyses were obtained from relative gray values of the protein bands (α-SMA/β-actin and FN/β-actin). (F,G) The result of qRT-PCR assay shows the α-SMA and FN mRNA level in MLg (α-SMA/GAPDH, and FN/GAPDH). Data were presented as mean ± SE, ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, compared with NC group. ^& p < 0.05, ^&& p < 0.01, compared with NC + TGF group. n = 3–5.