Figure 5.

Effect of Senp1-overexpression on H₂O₂ in HL-1 cells. (A) The effects of different concentrations of AS-IV (25, 50, 100 or 200 µmol/l) on the survival of HL-1 cells subjected to ISO. One-way ANOVA, followed by Tukey's test. (B) The expression of Senp1 protein was examined by Western blotting following HA-Senp1 plasmid transfection. T-test. (C) Compared with the control (dimethyl sulfoxide, 0.1%), H₂O₂ was increased in the 20 µmol/l ISO-induced HL-1 cells. AS-IV (50 µmol/l, n=6) prevented the ISO-induced increase in H₂O₂, which was inhibited by Senp1-overexpression (n=7). Data are presented as the mean ± standard deviation. Two-way ANOVA followed by Bonferroni's test. ^*P<0.05 vs. control; ^#P<0.05 vs. HF; ^&P<0.05 vs. HF+AS-IV. H₂O₂, hydrogen peroxide; AS-IV, astragaloside; ANOVA, analysis of variance; ISO, isoprenaline; Senp1, small ubiquitin-like modifier-specific protease 1.