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. 2021 Jul 28;22(4):1078. doi: 10.3892/etm.2021.10512

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Copyright: © Zhou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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E2F3 is a direct target gene of miR-195-5p. (A) TargetScan and ENCORI databases were used to predict the binding sequence between miR-195-5p and E2F3. (B) Dual-luciferase reporter assay was used to analyze the association between miR-195-5p and E2F3. (C) RT-qPCR was used to evaluate the effect of miR-195-5p overexpression on E2F3 expression. (D) RT-qPCR was used to evaluate the expression of E2F3 in AMC-HC-8 cells. ^*P<0.05; ^**P<0.01; and ^***P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; WT, wild type; MUT mutant.