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Stimulatory effects of HM and LPS on IL-1β mRNA expression in THP-1 cells. Representative electrophoresis gel showing the effects of increasing concentrations of HM (5-10 µg/ml. along with LPS (10 µg/ml. on the THP-1 cells, resulting in increased levels of IL-1β mRNA compared with the untreated cells after 3 h of incubation. β-actin mRNA was used as an internal loading control. HM, herbal melanin; LPS, lipopolysaccharide; IL-1β, interleukin-1β.
