Figure 5.

Effects of benazepril-HCl on activation of the Akt signaling pathway in DOX-treated H9c2 cells. H9c2 cells were treated with control or DOX (2 µM) in the presence or absence of benazepril-HCl for 24 h. t- and p-Akt protein levels were determined by (A) western blotting and (B) quantified as fold-changes compared with the control. Cells were pretreated with MK2206 and then exposed to DOX in the presence or absence of benazepril-HCl before DOX treatment for 24 h. Akt phosphorylation level was determined by (C) western blotting and (D) quantified with total Akt as a loading control. (E) Cell viability was assayed. (F) LDH release was detected. ^*P<0.05, ^**P<0.01. N=3 per group. DOX, doxorubicin; t-, total; p-, phosphorylated.