TABLE 1.
BEND3 and shSirt3-BEND3 cells were incubated in the absence (control) and presence of a combination of high glucose (30 mM) and palmitic acid (300 μM) for 48 h.
| PCR array of NF-κB target genes | ||||
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| ————BEND3———— | ————shSirt3-BEND3———— | |||
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| Control | HG + PA | Control | HG + PA | |
| Cox-2 | 1 | 124 | 2.4 | 256 |
| CXCL1 | 1 | 27.9 | 1.01 | 39.9 |
| RANTES | 1 | 6.1 | 1.8 | 15 |
| GADD45β | 1 | 6.8 | −1.14 | 10.9 |
| Adrenomedullin | 1 | 7.4 | −1.04 | 10.9 |
| CXCL10 | 1 | 2.2 | 1.2 | 5.5 |
| NQO1 | 1 | 3.0 | 1.6 | 3.1 |
| Myc | 1 | 2.6 | −1.05 | 2.4 |
| NF-κBIA | 1 | 2.3 | 1.2 | 2.3 |
The cells were processed for RNA isolation and pathway-specific gene expression array was performed. Fold inductions over BEND3-control are presented. shSirt3, Sirt3-silenced.