表 1.
Reverse transcription primers, upstream and downstream primers of real-time fluorescent quantitative PCR, inhibitor and mimics sequences for miR-381-3p
miR-381-3p 和 U6 的逆转录引物、实时荧光定量 PCR 上下游引物及 miR-381-3p 抑制物和模拟物序列
| 基因
Gene |
引物序列(5′→3′)
Primer sequence (5′→3′) |
| miR-381-3p | |
| 逆转录引物
Reverse transcription primer |
GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAGAG |
| 上游
Upstream |
CGCGTATACAAGGGCAAGCT |
| 下游
Downstream |
AGTGCAGGGTCCGAGGTATT |
| 抑制物
Inhibitor |
ACAGAGAGCUUGCCCUUGUAUA |
| 模拟物
Mimics |
UAUACAAGGGCAAGCUCUCUGU |
| 抑制物对照
NC inhibitor |
UCUACUCUUUCUAGGAGGUUGUGA |
| 模拟物对照
NC mimics |
UCACAACCUCCUAGAAAGAGUAGA |
| U6 | |
| 上游
Upstream |
CTCGCTTCGGCAGCACATA |
| 下游
Downstream |
ATTTGCGTGTCATCCTTGCG |