TABLE 1.
Genes associated with TMP susceptibility in E. coli K-12 W3110 strains.
Gene name | TMP MICs (μg ml–1) of wild-type strains | TMP MICs (μg ml–1) of gene deletion mutants | TMP MICs (μg ml–1) of complemented strains | TMP MICs (μg ml–1) of overexpressed strains |
mgrB | 0.16 | 1.28 | 0.16 | 0.16 |
ymjC | 0.16 | 0.32 | 0.32 | 0.16 |
rcsC | 0.16 | 0.32 | 0.16 | 0.16 |
tehB | 0.16 | 0.32 | 0.16 | 0.16 |
rfaH | 0.16 | 0.08 | 0.08 | 0.16 |
pgpA | 0.16 | 0.16 | 0.64 | 0.64 |
cysB | 0.16 | 0.64 | 0.16 | 0.16 |
torR | 0.16 | 0.64 | 0.64 | 0.16 |
phoP | 0.16 | 0.08 | 0.16 | 0.16 |
acrB | 0.16 | 0.04 | 0.08 | 0.16 |
yhfK | 0.16 | 0.16 | 0.08 | 0.08 |
acrA | 0.16 | 0.02 | 0.04 | 0.16 |
phoQ | 0.16 | 0.08 | 0.16 | 0.16 |
Cultures were incubated overnight at 37°C on LB with 10 μM IPTG.