TABLE 2.

MICs (μg ml^–1) to TMP in different strains of E. coli K-12 W3110.

Strain	Description	MIC
phoP/Q pathway-related strains
W3110 pCA24N	W3110 transformed with pCA24N	0.16
W3110 ΔmgrB ΔphoP pCA24N	W3110 ΔmgrB ΔphoP transformed with pCA24N	0.16
W3110 ΔmgrB ΔphoQ pCA24N	W3110 ΔmgrB ΔphoQ transformed with pCA24N	0.16
W3110 ΔmgrB pCA24N	W3110 ΔmgrB transformed with pCA24N	1.28
W3110 ΔphoP pCA24N	W3110 ΔphoP transformed with pCA24N	0.08
W3110 ΔphoQ pCA24N	W3110 ΔphoQ transformed with pCA24N	0.08
W3110 pCA24N:phoPQ	W3110 transformed with pCA24N:phoPQ	0.64
W3110 pCA24N:phoQ	W3110 transformed with pCA24N:phoQ	0.16
W3110 pCA24N:phoP	W3110 transformed with pCA24N:phoP	0.08
W3110 pCA24N:folA	W3110 transformed with pCA24N:folA	10.24
pmrK related strains
W3110 pCA24N	W3110 transformed with pCA24N	0.16
W3110 ΔpmrK pCA24N	W3110 ΔpmrK transformed with pCA24N	0.16
W3110 pCA24N:pmrK	W3110 transformed with pCA24N:pmrK	0.16
W3110 ΔpmrK pCA24N:pmrK	W3110 ΔpmrK transformed with pCA24N:pmrK	0.16
tolC related strains
W3110 pCA24N	W3110 transformed with pCA24N	0.16
W3110 pCA24N:tolC	W3110 transformed with pCA24N:tolC	0.16

Cultures were incubated overnight at 37°C on LB with 10 μM IPTG.