Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Selectable phenotype | Genotype and/or description | Source or reference |
|---|---|---|---|
| Mycobacterium smegmatis | |||
| None | M. smegmatis mc2155 △msmeg_1604 | This study | |
| None | M. smegmatis mc2155 △msmeg_5228 | This study | |
| HygR | M. smegmatis mc2155 △msmeg_1604/△ msmeg_5228 | This study | |
| HygR, KanR | M. smegmatis mc2155 △msmeg_1604/△ msmeg_5228 complemented with pMRLB121 | This study | |
| HygR, KanR | M. smegmatis mc2155 △msmeg_1604/△msmeg_5228 complemented with pMRLB122 | This study | |
| Escherichia coli | |||
| Top 10 | F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen | |
| Plasmid | |||
| pGEM®-T Easy Vector | AmpR | Cloning vector | Promega |
| pST-KT | KanR |
E. coli-mycobacteria shuttle vector Expression vector, UV15 promoter |
Addgene |
| pMRLB121 | KanR | pST-KT:ml1942 | This study |
| pMRLB122 | KanR | pST-KT:ml0389 | This study |