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Horizontal transmission is not observed when An. arabiensis larvae are reared in the same larval trough but separated by a screen mesh.
| Expt # | Number of | Microsporidia MB positives | Number of | Infection prevalence | Transmission |
| (Sheet labels) | donor larvae | in donor larvae | recipient larvae | in donor larvae | rate |
| LL1 | 10 | 8 | 14 | 0 | 0 |
| LL3 | 9 | 9 | 20 | 0 | 0 |
| LL4 | 14 | 4 | 31 | 0 | 0 |
| LL5 | 20 | 13 | 10 | 0 | 0 |
| LL6 | 7 | 7 | 9 | 0 | 0 |
