Figure 4.

Lifelong dietary ALA suppresses aging-linked thrombosis

(A and B) Representative images illustrating platelet adhesion over a vWF-coated microfluidic chamber under high-shear conditions (100 dyn/cm²) for 5 min in whole blood obtained from either young or old mice fed low (0.03%) or high (7.3%) doses of ALA for at least 16 months (n = 8), expressed as adherent platelet area/μm² of chamber surface at t = 5 min; Scale bar: 100 μm.

(C–E) Platelet activation analysis by flow cytometric analysis of platelet hyperreactivity in low or high-fed old mice (n = 8–9) in response to stimulation with low or high thrombin as determined by activation of platelet (C) GPIIb/IIIa and (D) GPIb, and (E) expression of surface P-selectin.

(F) Heatmap depicting plasma procoagulants TF and PAI-1 fold changes measured by multiplex proteome array in old mice fed a dietary low or high ALA for >16 months (n = 8–9) developed by chemiluminescence. All values are presented as mean ± S.D. ∗p < 0.05, ∗∗p < 0.01.