Figure 6.

LAMA4 expression does not change following HFD feeding reversal in mice. (A) Average weekly weights of mice on 45% HFD for 14 weeks (HFD) or 45% HFD for 8 weeks and then RCD for 6 weeks. HFD (n=3), Weight Loss (n=3), (**,***) indicates p < 0.01, 0.001, respectively. (B) Weight change shown as percentage from timepoint of diet switch (16 weeks of age) until end of dietary study (22 weeks of age). Data are Means ± SD. (C) Lama4 mRNA expression in sWAT of mice in HFD (n=3) or Weight Loss (n=3) group. (D) Relative Lama4 mRNA expression graphed against adipocyte area (um²) (n=6). Simple linear regression analysis. (E) Representative images of H&E stained sWAT tissue sections of HFD and Weight Loss group mice after 14 weeks of dietary study. All images are 40X magnification. (F) Adipocyte area (um²) in sWAT of mice in HFD (n=3) and Weight Loss (n=3) group.