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. 2021 Aug 3;36(5):109478. doi: 10.1016/j.celrep.2021.109478

Figure 7.

Figure 7

SETX or PRPF8 depletion triggers mtDNA R-loop accumulation and endogenous oxidative stress

(A) DRIP analyses in HeLa Kyoto cells after (si)RNA against SETX or PRPF8 for 48 h. Plots depict the mean ± SD from three independent experiments. The two-way ANOVA test was performed for all pairs to determine statistical significance. Statistically significant differences are indicated. ∗p < 0.05 and ∗∗∗p < 0.001.

(B) Fold change of the RNA level measure by qPCR with primers for D-loop region in HeLa Kyoto transfected with (si)RNA for (si)SETX or (si)PRPF8 compared with (si)Ctrl. Plots show the relative mean ± SD from three independent experiments. The two-tailed Student’s t test was performed to determine statistical significance between the two groups. ∗p < 0.05 and ∗∗p < 0.01.

(C and D) Bar graph showing relative ROS levels in HeLa Kyoto cells transfected for 72 h with (si)SETX (C), (si)PRPF8 (D), or (si)Ctrl. Intracellular ROS was measured as above using 5 μM of CM-H2DCFDA probe. The plot shows the mean ± SD from three independent experiments. The two-tailed Student’s t test was performed to determine statistical significance between groups. ∗∗p < 0.01.