FIGURE 5.

Pannexin labeling in mouse retina. (A) Immunostaining for Pannexin 1 in mouse retina revealed strong labeling in the outer plexiform layer and weaker labeling in the inner nuclear layer and ganglion cell layer. Three μm confocal stack. Labels for retinal nuclear layers are as in Figure 1. (B) Immunostaining for Pannexin 2 displayed strong labeling in the OPL and weaker labeling in the INL and photoreceptor layer. Three μm confocal stack. (C) Average mRNA expression levels of Pannexin1 and Pannexin2 in mouse retina from single-cell transcriptome data. In the OPL, the most prominent expression of Pannexin1 is in Off cone bipolar cells, with very weak expression in horizontal cells. Pannexin2 expression is largely absent from horizontal cells, but weakly present in On and Off bipolar cells. Data adapted from Hoang et al. (2020).