Table 2.
Schema for antagonist activity confidence scoring.
| Source | Criteria | Confidence Score Contribution |
|---|---|---|
| AR pathway model | AUC (Antagonist) ≥ 0.1 | 2 |
| 0.1 > AUC (Antagonist) ≥ 0.001 | 1 | |
| Cell stress/cytotoxicity flag | average Z-score > 3 | 2 |
| average Z-score < 1 | −1 | |
| Confirmation assay dataa | true antagonist shift (hit/hit) | 3 |
| true antagonist shift (no hit/hit) | 2 | |
| FLAG: true antagonist shift but CI overlap | 1 | |
| FLAG: wrong direction shift (hit/hit) | −1 | |
| FLAG: wrong direction (hit/no hit) | −1 | |
| Maximum receptor value | AUC (antagonist) < AUC (any other) | −1 |
| Cell-free assay activity (A1-A3) | A1-A3 are all inactive | −1 |
a
See the original AR model paper for more details (Kleinstreuer et al., 2017).