Figure 5. Genome-wide off-target analysis of prime editing.

(A) Schematic overview of the strategy to determine genome-wide off-target effects of prime editing. (B) Total amount of genome-wide single-nucleotide variant’s as determined by whole-genome sequencing. (C) Total amount of genome-wide indels as determined by whole-genome sequencing. (D) Mutational signature analysis by relative contribution of context-dependent mutation types in two controls and five prime-edited clonal organoid lines. (E) Integrative Genomics Viewer representation of a correct heterozygous prime editing–mediated mutation repair, a clone harboring an insertion downstream of the target site, and a clone with a deletion upstream of the target site.