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NMR backbone assignment of MS2–3C8 TCR β chains. (A) Overlaid two dimensional 1H-15N TROSY-HSQC spectra for MS2–3C8(mut1)α[β−2H, 15N] (black), MS2–3C8(mut2)α[β−2H, 15N] (green), and MS2–3C8(wt)α[β−2H, 15N] (red). Unassigned main chain signals are marked (x). (B) Expanded region highlighted (dashed box) in (A).
