Figure 4.

IFN-α treated monocytes showed enhanced peptide presentation to CD8⁺ T cells. (A–C) After CD14⁺ isolation, monocytes were incubated with IFNα overnight, loaded with different concentrations of gp100, washed, and gp100-specific CD8⁺ T cells were added. After 24h, IFNγ secretion by CD8⁺ T cells after co-culture with monocyte loaded with (B) short peptide or (C) long peptide was measured by ELISA. (D, E) Targeted antigen delivery to CD14⁺ CD169⁺ monocytes using antibody conjugated with gp100 peptide. (D) IFNα-treated CD14⁺ CD169^high monocytes or (E) freshly-isolated total CD14⁺ monocytes were loaded with different doses of αCD169-gp100 or control IgG-gp100 antibody-conjugates, washed, and gp100-specific CD8⁺ T cells were added. After 24h IFNγ secretion was measured by ELISA. Data are mean from four donors. Paired t-tests were used. *P < 0.05, **P < 0.01, ***P < 0.001.