Figure 3.

Mammospheres derived from the MD Anderson–metastatic breast cancer 231 mGFP or MD Anderson–metastatic breast cancer 231 angiopoietin-like protein 4 mGFP cell lines. (a) The number and morphology of ≥200 mm primary mammospheres. The mean number of MD Anderson–metastatic breast cancer 231 mGFP or MD Anderson–metastatic breast cancer 231 Angiopoietin-like protein 4-mGFP mammospheres ≥200 mm is 12 and 63, respectively, n = 3. The error bars depict the standard deviation. (b) Comparison of CD44 and cluster of differentiation 24 expression in adherent cells and mammospheres. (A = adherent, M = mammospheres). (c) Mammospheres stained with Oil Red O to detect triglycerides and cholesterol esters