Figure 4.

Anchorage-independent growth of cells derived from the MD Anderson– metastatic breast cancer 231 mGFP or MD Anderson–metastatic breast cancer 231 angiopoietin-like protein 4 mGFP cell lines, respectively. (a) The number and (b) morphology of ≥200 mm colonies derived from MD Anderson–metastatic breast cancer 231 mGFP or MD Anderson–metastatic breast cancer 231 angiopoietin-like protein 4 mGFP cells grown in suspension in semisolid medium containing 0.3% noble agar, respectively. The mean number of MD Anderson–metastatic breast cancer 231 mGFP or MD Anderson–metastatic breast cancer 231 angiopoietin-like protein 4 mGFP colonies ≥200 mm is 11 and 54, respectively, n = 3. The error bars depict the standard deviation