TABLE 2.

Details of 19 polymorphic miRNA-SSR markers used in the study of 20 terminal heat tolerant/susceptible wheat genotypes.

miRNA-SSR primer	SSR motif repeat	No. of alleles	Forward primer sequence (5′–3′)	Reverse primer sequence (5′–3′)	Tm (°C)	Amplicon size (bp)	PIC value
miR159	(TTCT)4	4	CTCACCCCTCTATAAAACGAC	CTACATCTATGGGGCTAGGAG	50	140–260	0.62
miR159b	(CTC)4	2	ATTTTCCTTTCAATGACACCT	AAGAGATGGAACGGAAACTAC	51	165–169	0.10
miR159c	(CT)27	3	CTTTCCCTCGTGCTTGGAT	GCATAGTGATTTGATTTTCTTGTTAGC	52	180–250	0.61
miR159f	(CT)27	3	ACCTGTATAGGTTTTGCATGA	TTAGGTGCAGACTGAAAACAT	51.5	130–162	0.62
miR164a	(GCCC)3	7	ACTGCACTGCACGTGTTCTT	TTGAAGACGCATACCTCGTG	59.5	135–315	0.81
miR165b	(ATAC)10	6	CAACGGTGTGATTGTAAAAA	CGAAGTTTAATTTGGTTATGC	50	146–280	0.72
mir171b	(ATTG)3	3	CTGAACGCTACTGAGCCACT	CTACCAACACGGCAGCACTA	55	178–300	0.65
mir172c	(TTC)5	3	CCTCTCTTTGTCTTCATCCA	AAGAACCGACTGTGATCTGA	51.5	130–180	0.25
mir393a	(GAGG)4	2	CCTATATAAGGACCTCACTGGA	AGGCATTGTTGCTCTCTCT	52	157–175	0.49
mir396d	(GTGC)3	6	AAGTTATATCGGACCGTGTG	AGGAAGGGGTCGTATAAATAG	52	150–470	0.79
mir404	(TGCCGC)3	2	CTAAACCGGATAAAGGGTAGA	CAGAGGAACGCACGTAGT	52	140–162	0.49
mir830	(AGGGA)3	9	AGTACGCCTTGATCTCCTCT	CTACGTTACCTTCCTCTCTCC	53.5	130–320	0.87
mir824	(TTAA)3	2	GGCCTTTGACTGAATTAGTGT	CCAGAAAGGAATTATTTTGGA	50	134–140	0.10
miR857	(TTTA)3	2	TTCTTGCCTACTTGTTTCTG	GTCGCCGTCTTTGAATTT	50	267–272	0.10
mir1130a	(TGCA)3	2	AGTTGCACTGCTACAAGCTAC	ACTTTCGGATGTACTGTTCTG	52	146–165	0.48
mir2102	(GCC)4	2	ACCGCTGCTGTTGTATTG	TCAAGTCCTCTGCAAACAC	52	167–175	0.48
mir2122	(TTCTT)3	2	GGGTGGACAGTAAAATCAGA	CCATTTTTCAGGATCATTCTT	50	157–162	0.10
mir5384	(CCG)4	3	AGGGGATCCTCCTCAGAT	AGGTGGTTGGTGGCCAAG	52	280–350	0.66
mir9662b	(CGC)5	2	CCTTCACCAAACCCTCTT	GAGATCCAGCAGAAGGAGAT	53	227–235	0.18