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Dual-electrode recordings. A: 10 sample traces of 100 before breaking in with the whole cell electrode. B: 10 sample traces of 100 after breaking in with whole cell electrode containing 5 mM Na+. No KNa channel activity was seen. C: a different cell with 10 sample traces before breaking in. D: by the 24th trace after breaking in with 5 mM Na+, channel activity appeared. Three channels were apparent, and two channels were constantly open. CA, cell-attached; WC, whole-cell.
