Comparisons of channel activity from excised inside-out patches to outside-out excised patches in the presence of 1 µM ouabain with defined concentrations of cytoplasmic Na+. A repeated measures two-way ANOVA was used to compare ISO and OSO values: F(1,34) = 0.16, P = 0.69, indicating no difference between the groups. ISO, inside-out; OSO, outside-out.