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. 2020 Aug 26;124(5):1377–1387. doi: 10.1152/jn.00097.2020

Fig. 8.

Fig. 8.

Micromolar nicotine did not increase shocked-evoked efferent transmitter release. A–E: number of evoked and spontaneous synaptic responses during 100 shocks at 1 Hz, repeated before and during perfusion with nicotine. F: exemplar recordings of postsynaptic currents evoked by short, facilitating shock trains in control and 1 µM nicotine. Circles correspond to values for individual recordings. Mean and standard deviation are presented for each condition. Control values are in black, nicotine values in gray. Paired two-tailed t tests were used to compare control versus experimental data A through F. A: effect of 0.3 µM nicotine (for n = 4 cells, no difference for control evoked versus nicotine evoked, P = 0.723, t = 0.337, df = 3, control spontaneous versus nicotine spontaneous P = 0.07, t = 2.74, df = 3). B: effect of 0.5 µM nicotine (for n = 2 cells, no difference for control evoked versus nicotine evoked, P = 0.685, t = 0.0538, df = 1, control spontaneous versus nicotine spontaneous P = 0.118, t = 5.32, df = 1). C: effect of 1 µM nicotine (for n = 3 cells, no difference for control evoked versus nicotine evoked, P = 0.245, t = 1.626, df = 2, control spontaneous versus nicotine spontaneous P = 0.593, t = 0.630, df = 2). D: effect of 10 µM nicotine (for n = 3 cells, no difference for control evoked versus nicotine evoked, P = 0.323, t = 1.302, df = 2, control spontaneous versus nicotine spontaneous P = 0.671, t = 0.493, df = 2). E: effect of 100 µM nicotine (for n = 4 cells, no difference for control evoked versus nicotine evoked, P = 0.076, t = 2.664, df = 3, control spontaneous versus nicotine spontaneous P = 0.589, t = 0.603, df = 3). F: example from one cell of the effect of 1 µM nicotine on a short (3-shock) train at 100 Hz to cause facilitated release from the efferents, recorded at −94 mV. Black is the average of 5 control responses from the exemplar cell, gray is the average of 5 responses in nicotine from that same cell. For n = 6 cells the average peak amplitude in control (50.8 pA, ± 40.5 SD) versus nicotine (36.1 pA, ± 24.9 SD) was not different (P = 0.1205, t = 1.869, df = 5).