FIGURE 5.

Expression of P. phymatum wild-type GFP reporter constructs (pPROBE, p5978, p6115, and p6116) in nodules of Phaseolus vulgaris (A) and Mimosa pudica (B). The pPROBE was used as negative control. Images were acquired with a confocal laser scanning microscope (DM5500Q; Leica) and single cells specific fluorescence quantified with Fiji. Plus signs indicate the arithmetic mean, whiskers minimum to maximum values and black dots outliers according to Tukey’s test. Significant differences between samples were analyzed in a one-way ANOVA with Tukey’s post hoc test (^****p-value < 0.0001, ns, not significant). All T6SS reporter constructs values were found statistically different from pPROBE values.