TABLE 1.

The symbiotic properties (nodules per plant, weight per nodule, and nitrogenase activity) of P. vulgaris and M. pudica nodules infected by P. phymatum wild-type, ΔT6SS-b, ΔT6SS-3, and ΔΔT6SS.

Strain	Plant	Number of nodules	Nodule dry weight (mg)	Weight per nodule (mg)	Nitrogen fixation (%/g/min)
WT	P. vulgaris	109 ± 38	11.96 ± 3.25	0.11 ± 0.02	0.06 ± 0.04
ΔTssBC-3	P. vulgaris	82 ± 29	8.74 ± 2.37	0.12 ± 0.05	0.13 ± 0.06
ΔTssBC-b	P. vulgaris	110 ± 11	12.85 ± 1.85	0.12 ± 0.01	0.09 ± 0.04
ΔΔTssBC	P. vulgaris	103 ± 38	10.28 ± 3.64	0.09 ± 0.03	0.07 ± 0.06
WT	M. pudica	5 ± 2	0.48 ± 0.13	0.13 ± 0.08	1.34 ± 0.30
ΔTssBC-3	M. pudica	5 ± 2	0.45 ± 0.09	0.10 ± 0.03	1.43 ± 0.38
ΔTssBC-b	M. pudica	6 ± 2	0.47 ± 0.19	0.09 ± 0.02	1.25 ± 0.32
ΔΔTssBC	M. pudica	6 ± 1	0.53 ± 0.05	0.09 ± 0.02	1.29 ± 0.26

Two biological replicates, consisting of five plants per replicate, were used (n = 10) for each strain. Values indicate the average and the standard deviation.