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. Author manuscript; available in PMC: 2021 Oct 5.
Published in final edited form as: Dev Cell. 2021 Mar 17;56(7):1014–1029.e7. doi: 10.1016/j.devcel.2021.02.025

Figure 3. Loss of NELF in the regenerating muscle leads to a depletion of the MuSC pool.

Figure 3.

(A) Experimental schematic showing the sequential injury approach where mice that had undergone tamoxifen-induced recombination were subjected to two rounds of CTX treatment at 28-day intervals.

(B) Hematoxylin and eosin stain of the regenerated TA muscle after the repeat injury (±SE, p-value< 0.001, n = 3) was used to visualize regeneration and the weight of the regenerated muscle was determined relative to the uninjured contralateral TA muscle (±SE, n = 3), scale bar = 1 mm.

(C) Magnification of the hematoxylin and eosin staining for the repeat-injured TA from (B) showing fibrosis and interstitial cells, scale bar = 50 μm.

(D) Immunofluorescence characterization of MuSCs (Pax7+) in the regenerated TA muscle 7 or 28 days after injury (±SE, p-value < 0.001, n = 4), scale bar = 25 μm.