KEY RESOURCES TABLE

Reagent or resource	Source	Identifier
Antibodies
Rabbit polyclonal anti-HA	Novus Biologicals	Cat#NB600-363; RRID: AB_10001504
Rabbit polyclonal anti-ETV4	Proteintech	Cat#10684-1-AP; RRID: AB_2100984
Rabbit polyclonal anti-DNMT3L	Abcam	Cat#ab194094; RRID: AB_2783649
Rabbit polyclonal anti-DNMT3L	A gift from Dr. Shinya Yamanaka (Kyoto University, Japan)	N/A
Rat monoclonal anti-GCNA1 (TRA98)	B-Bridge International	Cat# 73-003; RRID: AB_1056334
Guinea Pig polyclonal anti-IgG	Antibodies-Online	Cat# ABIN101961; RRID: AB_10775589
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Thermo Fisher Scientific	Cat# A-11008; RRID: AB_143165
Goat anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 555 conjugate	Thermo Fisher Scientific	Cat# A-21434; RRID: AB_141733
Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647	Thermo Fisher Scientific	Cat# A-31573; RRID: AB_2536183
Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594	Thermo Fisher Scientific	Cat# A-21209; RRID: AB_2535795
Bacterial and virus strains
pGL3 vector	Promega	Cat# E1771
pRL-cmv vector	Promega	Cat# E2261
pLLU2G vector	Addgene	Cat#21620; RRID:Addgene_21620
pCAG-ERT2CreERT2	Addgene	Cat#13777; RRID:Addgene_13777
Biological samples
Testis blocks	Mouse testes	N/A
Postnatal day (P) 0 – P2 testes	Mouse testes	N/A
Chemicals, peptides, and recombinant proteins
Human, FGF-Basic, recombinant	Sigma-Aldrich	Cat# F0291-25UG
Collagenase type IV	Worthington Biochemical	Cat# LS004212
Deoxyribonuclease I	Worthington Biochemical	Cat# LS006355
(Z)-4-Hydroxytamoxifen	Sigma-Aldrich	Cat# H7904
4-Thiouridine	Millipore Sigma	Cat# T4509
Protein A-Tn5 transposase fusion protein	A gift from Dr. Steven Henikoff (Fred Hutchinson Cancer Research Center)	N/A
Critical commercial assays
iScript cDNA synthesis Kit	BioRad	Cat# 170-8891
SsoAdvanceD Universal SYBR Green Supermix	BioRad	Cat# 172-5274
Dual Luciferase assay kit	Promega	Cat# E1960
QuantSeq 3′ mRNA-Seq Library Prep Kit	Lexogen	Cat# 015.24
NEBNext Ultra II Directional RNA Library Prep kit	New England Biolabs	Cat# E7765S
Deposited data
RNaseq raw and analyzed data	Generated in this study	GEO: GSE165372
CUT&Tag raw and analyzed data	Generated in this study	GEO: GSE165372
iSLAMseq raw and analyzed data	Generated in this study	GEO: GSE165372
Mus musculus, release-96, Ensembl genome (GRCm38)	Ensembl genome	http://ftp.ensembl.org/pub/release-96/
Mus musculus (mm10) genome	UCSC	https://hgdownload.soe.ucsc.edu/downloads.html#mouse
Experimental models: Cell lines
Mouse: GC1 cells	ATCC	CRL-2053
Human: HEK293T	ATCC	CRL-3216
Human: TCam-2 cells	de Jong et al., 2008	RRID: CVCL_T012
Experimental models: Organisms/strains
Mouse: C57BL/6J	The Jackson Laboratory	JAX:000664
Mouse: B6.129-Rhox10tm1Wilk/J	The Jackson Laboratory	JAX: 035193
Mouse: B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J	The Jackson Laboratory	JAX:004654
Oligonucleotides
Oligonucleotides designed in this study	See Table S3 for oligonucleotide information	N/A
Software and algorithms
Bowtie2 (v2.2.5)	Langmead and Salzberg, 2012	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
Samtools	Li et al., 2009	http://samtools.sourceforge.net/
Homer	Heinz et al., 2010	http://homer.ucsd.edu/homer/
IGV (version 2.6.2)	Robinson et al., 2011	https://software.broadinstitute.org/software/igv/
STAR (2.5.2b)	Dobin and Gingeras, 2015	https://github.com/alexdobin/STAR
SubRead	Liao et al., 2013	http://subread.sourceforge.net/
SLAM-DUNK (v0.4.3)	Neumann et al., 2019	https://github.com/t-neumann/slamdunk
R Version 4.0.0	https://www.r-project.org	N/A
DESeq2	Love et al., 2014	N/A
limma (v3.42.2)	Ritchie et al., 2015	N/A
GraphPad Prism (v9.0)	N/A	https://www.graphpad.com/scientific-software/prism/
The database for annotation, visualization and integrated discovery (DAVID) v6.8	Huang et al., 2009	https://david.ncifcrf.gov/