Fig. 4.

(a) Representative cross-sectional FLIm LT and intensity ratio images of cartilage without treatment and cartilage treated with high ($2\mathrm{U}/\mathrm{mL}$) and low ($1\mathrm{U}/\mathrm{mL}$) concentration of cABC. The depleted region is segmented using channel 1 intensity ratio of 0.25. $\text{Scalebar}=1\mathrm{mm}$. (b) Box plot of the thickness of the GAG depleted layer calculated from segmentation result **$p<0.01$ ($n\ge 6$ per treatment group).