FIG 6.

Identification of HspR target genes involved in H₂O₂ stress response. (A) Sensitivity of WT and ΔhspR strains to various stress conditions. Spore suspensions were diluted serially and spotted on YMS agar containing NaCl, KCl, TBHP, H₂O₂, or diamide at the indicated concentrations. (B) Promoter probes of H₂O₂ stress-related genes used in EMSAs. (C) EMSAs of His₆-HspR with indicated probes. Notations as in Fig. 2D. (D) ChIP-qPCR assays of HspR binding to katA2p, katA3p, catR_katA1, and oxyR_ahpCD. Notations as in Fig. 2E. (E) RT-qPCR analysis of six HspR target H₂O₂ stress-related genes in WT and ΔhspR strains grown in FM-I. Error bars (panels D and E) indicate SDs from three replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (t test).