FIG 2.

Phylogenetic tree based on 16S rRNA gene for Acidobacteria classes. Groups other than family UBA6911 (subgroup 18) are shown as gray wedges. A total of 177 near-full-length (>1,200-bp) 16S rRNA sequences belonging to Acidobacteria group 18 in the SILVA database are shown with their GenBank accession numbers. Representative 16S rRNA sequences from the analyzed genomes are shown with their corresponding bin name (as in Table 1) and with a colored dot next to the name for ease of recognition. Branch labels are color coded by genus, as shown in the legend (using the same color scheme as that in Fig. 1). The track around the tree shows the environmental classification of the ecosystem from which the sequence was obtained, and the corresponding color codes are shown in the legend. Sequences were aligned using the SINA aligner (97), and the alignment was used to construct a maximum likelihood phylogenetic tree with FastTree (92). Bootstrap values (from 100 bootstraps) are displayed as bubbles for branches with ≥70% support. The tree was rooted using Chloroflexus aggregans (GenBank accession number M34116.1) as the outgroup.