FIG 2.

Growth of M. maripaludis strain JJ and mutants in standing culture with H₂ as the electron donor. (A) Quantification of surface-associated biomass from cultures grown in McCas medium with H₂ provided as the sole electron donor and stained with crystal violet. Cultures were grown without agitation for 96 h, the amount of time needed to reach stationary phase. Data are averages and standard deviations from four independent experiments. (B) Surface-attached biomass from microscope coverslips submerged in McCas medium with H₂ provided as the sole electron donor. Images were taken from the center of the coverslip near the area corresponding to the atmosphere-liquid interface. Bar = 50 μm. ***, P < 0.001.