FIG 4.

Glycosylation of the S-layer protein of M. maripaludis strain JJ. Alignment of S-layer proteins SlpA (A) and SlpB (B). Black lines indicate positions of amino acid identity and putative N-linked glycosylation sites (N-X-S/T, where X is any amino acid other than proline) are indicated in green. (C) Coomassie stained SDS-PAGE gel of the supernatant from whole cells that were suspended in protoplasting buffer. SlpA proteins were identified by mass spectrometry. (D) Periodic acid-Schiff stained SDS-PAGE gel showing the presence of glycoproteins.