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. Author manuscript; available in PMC: 2021 Aug 11.
Published in final edited form as: Nat Aging. 2021 Feb 11;1(2):205–217. doi: 10.1038/s43587-021-00027-5

Extended Data Fig. 6. The effects of SIRT3 overexpression on FoxO3a recovery in TGF-β1 treated human lung fibroblasts.

Extended Data Fig. 6

(a) Left panel, schematic of the experiment design. Right panel, representative western blot showing levels of HA tag, SIRT3, FoxO3a and α-SMA in human fibroblasts cells transfected with SIRT3-HA plasmid followed by treatment with TGF-β1 (2.5 ng/ml) for 48 hours. (b) Left panel, schematic diagram of the experiment design. Right panel, representative western blot analyses of HA, SIRT3, FoxO3a and α-SMA in IMR-90 cells treated with TGF-β1 for 48 hours followed by SIRT3 overexpression.