Skip to main content
. Author manuscript; available in PMC: 2021 Aug 11.
Published in final edited form as: Nat Aging. 2021 Feb 11;1(2):205–217. doi: 10.1038/s43587-021-00027-5

Figure 7: SIRT3 overexpression in macrophages activates FoxO3a in fibroblasts via paracrine signaling mechanism.

Figure 7:

(a) Experimental design of studies of SIRT3-HA overexpression in IPF fibroblasts. (b) Representative western blots showing SIRT3, HA and FoxO3a levels in cytoplasmic and nuclear fractions of IPF fibroblasts (n = 3 different IPF subjects) overexpressing control or SIRT3-HA plasmid; c -cytoplasm, n -nuclear fraction. (c) Design of co-culture studies of SIRT3-HA overexpressing RAW264.7 macrophages and lung fibroblasts from young or old mice; WCE -whole cell lysates. (d-f) Western blots showing SIRT3 levels in whole cell lysates of RAW264.7 (d) or FoxO3a levels in the cytoplasm (e), or FoxO3a levels in nuclear fractions (f) from young and old mouse fibroblasts. (g) Western blots showing expression levels of cleaved caspase-3, cleaved PARP, Bcl-2, Bim and Bad in cytoplasmic fractions from young and old mouse fibroblasts in co-culture with RAW264.7 mouse macrophages, as depicted in (c). Co-culture experiments were performed twice with each lane of the western blots representing pooled wells from each 6-well plate.