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. 2021 Jul 28;12:706758. doi: 10.3389/fmicb.2021.706758

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Copyright © 2021 Wang, Huang, Wang, Yang, He, Liu, Tu and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of dietary supplementation with herbal extract mixture (HEM) on the Nrf2–Keap1 pathway in weaning pigs. Relative mRNA expression of Keap1 (A) and Nrf2 (B) in the jejunum and the ileum was measured by quantitative real-time PCR. Relative protein expression and quantitative analysis of Keap1 (C) and Nrf2 (D) in the jejunum and the ileum was measured by Western blotting. CON, basal diet; HEM, basal diet supplemented with 1,000 mg/kg herbal extract mixture. Data are presented as mean ± SEM, n = 9. *p < 0.05. 0.01 < **p < 0.05.