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. Author manuscript; available in PMC: 2021 Aug 11.
Published in final edited form as: J Vis Exp. 2018 Nov 9;(141):10.3791/58310. doi: 10.3791/58310

Figure 2: Murine bronchoalveolar lavage fluid-derived EVs isolated by ultrafiltration centrifugation and ultracentrifugation with density gradient centrifugation methods expressed tetraspanin proteins CD63, CD9, and CD81.

Figure 2:

Shown are percentages of EVs stained positive by the UFC and UC-DCG isolation techniques, illustrated by pseudocolor plots, for (A - C) PE-CD63, (D - F) FITC-CD9, and (G - I) PE-CD9. The data are derived from three independent experiments. UFC = ultrafiltration centrifugation; UC-DGC = ultracentrifugation with density gradient centrifugation; EVs = extracellular vesicles; SSC-A = side scatter analysis; PE = phycoerythrin; FITC = fluorescein isothiocyanate. Please click here to view a larger version of this figure.