Table 1: Buoyant density gradient buffers.

This table gives the composition and buffer ratio of each gradient solution that was used to purify murine bronchoalveolar lavage fluid-derived extracellular vesicle populations isolated by the ultracentrifugation technique.

Working Solution Iodixanol (%)	5	15	25	30
Working solution (mL) *	2	6	10	12
Homogenization medium (mL) **	18	14	10	8

^*The working solution was 50% iodixanol (25 mL of density gradient medium [see Table of Materials] + 5 mL of diluent solution [pH 7.4 of 0.25 M sucrose + 120 mM HEPES + 0.9 M NaCl]).

^**Homogenization medium (pH 7.4 of 0.25 M sucrose + 20 mM HEPES + 150 mM NaCl)